Genotyping and primer details


Genotyping and primer details for SALK_048982c - At3g05200
Line ID SALK_048982c
Locus At3g05200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1477303 of line SALK_048982c Chr3:1477303
   
Insertion allele amplicon LBb3 - 24kw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24kw: 5'-GTAATCTCCTCGAATCGACTTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1478046
   
wt amplicon (over InsPos) 07px - 24kw
wt amplicon start Chr3:1477194
wt amplicon end Chr3:1478046
wt amplicon size 853 bp
Locus specific primer 24kw: 5'-GTAATCTCCTCGAATCGACTTCAC-3'
2nd locus specific primer 07px: 5'-ACATATTTGTTGATTGGAGGAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11