Genotyping and primer details


Genotyping and primer details for 146E09 - At3g06010
Line ID 146E09
Locus At3g06010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1804542 of line 146E09 Chr3:1804542
   
Insertion allele amplicon 8474 - DF16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DF16: 5'-GGTTTGGGAAAAACTATTCAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1804916
   
wt amplicon (over InsPos) 11fw - DF16
wt amplicon start Chr3:1804065
wt amplicon end Chr3:1804916
wt amplicon size 852 bp
Locus specific primer DF16: 5'-GGTTTGGGAAAAACTATTCAAACA-3'
2nd locus specific primer 11fw: 5'-TCCTTCTCAGTATAAACGGCCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11