Genotyping and primer details


Genotyping and primer details for SALK_035240c - At3g06830
Line ID SALK_035240c
Locus At3g06830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2154709 of line SALK_035240c Chr3:2154709
   
Insertion allele amplicon LBb3 - 09gj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09gj: 5'-TTGGAGATTGAAACTCACTGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2155318
   
wt amplicon (over InsPos) 07ho - 09gj
wt amplicon start Chr3:2154289
wt amplicon end Chr3:2155318
wt amplicon size 1030 bp
Locus specific primer 09gj: 5'-TTGGAGATTGAAACTCACTGATTG-3'
2nd locus specific primer 07ho: 5'-TCTAAACACTCTCCCAAAGACGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11