Genotyping and primer details


Genotyping and primer details for 349H10 - At3g47580
Line ID 349H10
Locus At3g47580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17534084 of line 349H10 Chr3:17534084
   
Insertion allele amplicon 8474 - EQ29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ29: 5'-CTGCTACCAAACATTCGTGAGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17533455
   
Insertion allele amplicon 8474 - EQ30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ30: 5'-TGTCTTTCAAGGATTCACATTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17534949
   
wt amplicon (over InsPos) EQ30 - EQ29
wt amplicon start Chr3:17533455
wt amplicon end Chr3:17534949
wt amplicon size 1495 bp
Locus specific primer EQ29: 5'-CTGCTACCAAACATTCGTGAGTTA-3'
2nd locus specific primer EQ30: 5'-TGTCTTTCAAGGATTCACATTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11