Genotyping and primer details


Genotyping and primer details for SALK_076027c - At3g50900
Line ID SALK_076027c
Locus At3g50900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18918319 of line SALK_076027c Chr3:18918319
   
Insertion allele amplicon LBb3 - 21gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21gg: 5'-AAGCTAAGGTGGGAAATGAAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18918824
   
wt amplicon (over InsPos) 08ho - 21gg
wt amplicon start Chr3:18917840
wt amplicon end Chr3:18918824
wt amplicon size 985 bp
Locus specific primer 21gg: 5'-AAGCTAAGGTGGGAAATGAAGAAT-3'
2nd locus specific primer 08ho: 5'-CGATTTAATAGGGACGAACAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11