Genotyping and primer details


Genotyping and primer details for 382H01 - At3g54250
Line ID 382H01
Locus At3g54250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20084590 of line 382H01 Chr3:20084590
   
Insertion allele amplicon 3144 - FS09
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FS09: 5'-AGTTCATGCATCACAATGATTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20084957
   
Insertion allele amplicon 8474 - HV14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HV14: 5'-TCATCAACAGAGAGAAAAAGCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20084148
   
wt amplicon (over InsPos) FS09 - HV14
wt amplicon start Chr3:20084148
wt amplicon end Chr3:20084957
wt amplicon size 810 bp
Locus specific primer HV14: 5'-TCATCAACAGAGAGAAAAAGCAGA-3'
2nd locus specific primer FS09: 5'-AGTTCATGCATCACAATGATTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11