Genotyping and primer details


Genotyping and primer details for SALK_024296 - At3g56580
Line ID SALK_024296
Locus At3g56580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20962701 of line SALK_024296 Chr3:20962701
   
Insertion allele amplicon LBb3 - 44et
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44et: 5'-TAATTCGATTTTCTCTCCATTGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20962028
   
wt amplicon (over InsPos) 11gc - 44et
wt amplicon start Chr3:20962028
wt amplicon end Chr3:20962852
wt amplicon size 825 bp
Locus specific primer 44et: 5'-TAATTCGATTTTCTCTCCATTGCT-3'
2nd locus specific primer 11gc: 5'-ATCAATAGAGGAGCCAAGTTTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11