Genotyping and primer details


Genotyping and primer details for 491A12 - At4g13000
Line ID 491A12
Locus At4g13000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7598329 of line 491A12 Chr4:7598329
   
Insertion allele amplicon 8474 - HF40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HF40: 5'-AAAGAAGGCCAAAGATGAGTACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7599050
   
wt amplicon (over InsPos) 07fd - HF40
wt amplicon start Chr4:7598026
wt amplicon end Chr4:7599050
wt amplicon size 1025 bp
Locus specific primer HF40: 5'-AAAGAAGGCCAAAGATGAGTACAA-3'
2nd locus specific primer 07fd: 5'-GGCCAGTTTGACAATTACCTAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11