Genotyping and primer details


Genotyping and primer details for 815B08 - At4g16150
Line ID 815B08
Locus At4g16150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9149746 of line 815B08 Chr4:9149746
   
Insertion allele amplicon 8474 - MK18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MK18: 5'-CGAAGGTGTTACTGGTTATTGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9149274
   
wt amplicon (over InsPos) 03id - MK18
wt amplicon start Chr4:9149274
wt amplicon end Chr4:9150340
wt amplicon size 1067 bp
Locus specific primer MK18: 5'-CGAAGGTGTTACTGGTTATTGGAT-3'
2nd locus specific primer 03id: 5'-GACTAGAAAATTCTCCTGCACCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11