Genotyping and primer details


Genotyping and primer details for 250G03 - At5g08750
Line ID 250G03
Locus At5g08750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2854315 of line 250G03 Chr5:2854315
   
Insertion allele amplicon 8474 - KM62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KM62: 5'-TCAGAGACACCAACTTGAAAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2855026
   
wt amplicon (over InsPos) 21si - KM62
wt amplicon start Chr5:2853951
wt amplicon end Chr5:2855026
wt amplicon size 1076 bp
Locus specific primer KM62: 5'-TCAGAGACACCAACTTGAAAAGAA-3'
2nd locus specific primer 21si: 5'-CGTGTCGGAAAGGTATGTATGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11