Genotyping and primer details


Genotyping and primer details for SALK_016289c - At5g22300
Line ID SALK_016289c
Locus At5g22300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7381076 of line SALK_016289c Chr5:7381076
   
Insertion allele amplicon LBb3 - 01cw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 01cw: 5'-CTAATAAATTCTCACCAAAGCCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7380343
   
wt amplicon (over InsPos) 23dn - 01cw
wt amplicon start Chr5:7380343
wt amplicon end Chr5:7381496
wt amplicon size 1154 bp
Locus specific primer 01cw: 5'-CTAATAAATTCTCACCAAAGCCGT-3'
2nd locus specific primer 23dn: 5'-AGTTTGGTCCAGCTAAAACAATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11