Genotyping and primer details


Genotyping and primer details for 509E02 - At5g63030
Line ID 509E02
Locus At5g63030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25286765 of line 509E02 Chr5:25286765
   
Insertion allele amplicon 8474 - SZ15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SZ15: 5'-TCACCTGAAGTAAACATAGCATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25287238
   
wt amplicon (over InsPos) 06cb - SZ15
wt amplicon start Chr5:25286252
wt amplicon end Chr5:25287238
wt amplicon size 987 bp
Locus specific primer SZ15: 5'-TCACCTGAAGTAAACATAGCATCG-3'
2nd locus specific primer 06cb: 5'-GAGATAAAAACTTCATTTGCCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11