Genotyping and primer details


Genotyping and primer details for 330F08 - At5g65550
Line ID 330F08
Locus At5g65550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26199707 of line 330F08 Chr5:26199707
   
Insertion allele amplicon 8474 - PE54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PE54: 5'-CCCATCAGGTAGTAGCATAGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26198833
   
wt amplicon (over InsPos) 08lt - PE54
wt amplicon start Chr5:26198833
wt amplicon end Chr5:26200271
wt amplicon size 1439 bp
Locus specific primer PE54: 5'-CCCATCAGGTAGTAGCATAGAAGC-3'
2nd locus specific primer 08lt: 5'-AAGTGAGGACAAAAGGATTTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11