Genotyping and primer details


Genotyping and primer details for 011E08 - At5g66710
Line ID 011E08
Locus At5g66710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26637229 of line 011E08 Chr5:26637229
   
Insertion allele amplicon 3144 - DE20
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer DE20: 5'-GAAGAGTATTGCCTTCCATGAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26637594
   
Insertion allele amplicon 8474 - FI01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FI01: 5'-ATCAATTCGCTTTCACTATCAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26636769
   
wt amplicon (over InsPos) DE20 - FI01
wt amplicon start Chr5:26636769
wt amplicon end Chr5:26637594
wt amplicon size 826 bp
Locus specific primer FI01: 5'-ATCAATTCGCTTTCACTATCAACA-3'
2nd locus specific primer DE20: 5'-GAAGAGTATTGCCTTCCATGAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11