Genotyping and primer details


Genotyping and primer details for 016D02 - At1g18130
Line ID 016D02
Locus At1g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6238534 of line 016D02 Chr1:6238534
   
Insertion allele amplicon 8474 - 11bt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11bt: 5'-TTACCTGGTTTTGTTAGGGTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6238966
   
wt amplicon (over InsPos) 07da - 11bt
wt amplicon start Chr1:6238086
wt amplicon end Chr1:6238966
wt amplicon size 881 bp
Locus specific primer 11bt: 5'-TTACCTGGTTTTGTTAGGGTTTTG-3'
2nd locus specific primer 07da: 5'-ATATCAGGTTGATTTTCACCTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11