Genotyping and primer details


Genotyping and primer details for 016H10 - At5g01900
Line ID 016H10
Locus At5g01900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:351501 of line 016H10 Chr5:351501
   
Insertion allele amplicon 3144 - 58wf
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 58wf: 5'-CATCCGATAGAGAGGTTAACGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:352223
   
Insertion allele amplicon 3144 - FM76
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FM76: 5'-AGAAAGACATCTATGGCAGAGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:351663
   
Insertion allele amplicon 8474 - IS16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS16: 5'-CTCATGCTTAAATGCGTACCATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:350693
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11