Genotyping and primer details


Genotyping and primer details for 021C04 - At1g77300
Line ID 021C04
Locus At1g77300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29041071 of line 021C04 Chr1:29041071
   
Insertion allele amplicon 8474 - PP12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP12: 5'-CTGTTTGTTGGGACAAGATAGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29040681
   
wt amplicon (over InsPos) AJ14 - PP12
wt amplicon start Chr1:29040681
wt amplicon end Chr1:29041480
wt amplicon size 800 bp
Locus specific primer PP12: 5'-CTGTTTGTTGGGACAAGATAGTCA-3'
2nd locus specific primer AJ14: 5'-AGTCATCCGACCAGATATTGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11