Genotyping and primer details


Genotyping and primer details for 022G12 - At2g15300
Line ID 022G12
Locus At2g15300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6651547 of line 022G12 Chr2:6651547
   
Insertion allele amplicon 8474 - KE43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE43: 5'-AAACGAACAACAATTCAAATGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6652095
   
wt amplicon (over InsPos) 03cj - KE43
wt amplicon start Chr2:6650986
wt amplicon end Chr2:6652095
wt amplicon size 1110 bp
Locus specific primer KE43: 5'-AAACGAACAACAATTCAAATGACA-3'
2nd locus specific primer 03cj: 5'-ACTCAACTAGTGACAGTGGATGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11