Genotyping and primer details


Genotyping and primer details for 027F07 - At5g36290
Line ID 027F07
Locus At5g36290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14304429 of line 027F07 Chr5:14304429
   
Insertion allele amplicon 8474 - RK74
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK74: 5'-TATTTGACATGGTGAAATGGTAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14304030
   
Insertion allele amplicon 8474 - RK75
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK75: 5'-CCTCCAACAACTGCTAGTGAAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14304869
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11