Genotyping and primer details


Genotyping and primer details for 030C06 - At5g16820
Line ID 030C06
Locus At5g16820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5532123 of line 030C06 Chr5:5532123
   
Insertion allele amplicon 3144 - FL53
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FL53: 5'-CTTCATATCTCCTTTTGCAACCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5532614
   
Insertion allele amplicon 8474 - RE34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RE34: 5'-AACAAATGATGTCGTTTTTAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5531632
   
wt amplicon (over InsPos) FL53 - RE34
wt amplicon start Chr5:5531632
wt amplicon end Chr5:5532614
wt amplicon size 983 bp
Locus specific primer RE34: 5'-AACAAATGATGTCGTTTTTAGCAA-3'
2nd locus specific primer FL53: 5'-CTTCATATCTCCTTTTGCAACCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11