Genotyping and primer details


Genotyping and primer details for 034B01 - At2g26670
Line ID 034B01
Locus At2g26670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11342220 of line 034B01 Chr2:11342220
   
Insertion allele amplicon 8474 - MR74
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR74: 5'-TTAGCTCCGATTTCTTCATCCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11341825
   
wt amplicon (over InsPos) FL62 - MR74
wt amplicon start Chr2:11341825
wt amplicon end Chr2:11342674
wt amplicon size 850 bp
Locus specific primer MR74: 5'-TTAGCTCCGATTTCTTCATCCTTA-3'
2nd locus specific primer FL62: 5'-ATTCATACAAAACACCACAACCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11