Genotyping and primer details


Genotyping and primer details for 043D04 - At4g29340
Line ID 043D04
Locus At4g29340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14447483 of line 043D04 Chr4:14447483
   
Insertion allele amplicon 8474 - CL48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CL48: 5'-ACTGAACTCCTGTCCCTTGAACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14448130
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 31.05.2018 12:55