Genotyping and primer details


Genotyping and primer details for 052D02 - At5g09860
Line ID 052D02
Locus At5g09860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3070592 of line 052D02 Chr5:3070592
   
Insertion allele amplicon 3144 - AM28
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AM28: 5'-TTGATAGGTTTACTGCTGGAAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3070215
   
Insertion allele amplicon 3144 - CW46
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CW46: 5'-TTGATAGGTTTACTGCTGGAAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3070215
   
Insertion allele amplicon 3144 - CW47
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CW47: 5'-GTGACACGACTTAAAAACCACTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3071149
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11