Genotyping and primer details


Genotyping and primer details for 053G05 - At3g27650
Line ID 053G05
Locus At3g27650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10239662 of line 053G05 Chr3:10239662
   
Insertion allele amplicon 8474 - CW41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW41: 5'-GAGACAGAGAGTGCTTTGATGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10239138
   
Insertion allele amplicon 8474 - CW42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW42: 5'-TACCTCATGAGATCAGCATTTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10240291
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11