Genotyping and primer details


Genotyping and primer details for 055G12 - At1g50325
Line ID 055G12
Locus At1g50325 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18640198 of line 055G12 Chr1:18640198
   
Insertion allele amplicon 8474 - 02yg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02yg: 5'-CTTTTTCTGGTTCTTCTAGCACCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18639283
   
Insertion allele amplicon 8474 - 03wj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03wj: 5'-AATCTCAAATCCCAAGTTTGTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18638994
   
Insertion allele amplicon 8474 - 14hj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14hj: 5'-TGGTGGATTTCGATTTTTCTTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18639788
   
Insertion allele amplicon 8474 - 16yg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16yg: 5'-CATGACCATTAGAATTACCTAGGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18641693
   
Insertion allele amplicon 8474 - 36yk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36yk: 5'-TATCAAACCTGAGATAAGGCTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18639115
   
Insertion allele amplicon 8474 - pg01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer pg01: 5'-ATTCTCCAGCAAGAACTGATGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18639072
   
wt amplicon (over InsPos) 33si - 14hj
wt amplicon start Chr1:18639788
wt amplicon end Chr1:18640492
wt amplicon size 705 bp
Locus specific primer 14hj: 5'-TGGTGGATTTCGATTTTTCTTAAC-3'
2nd locus specific primer 33si: 5'-TTGAAAAACAAAAATGGTTGCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 02.09.2016 15:25