Genotyping and primer details


Genotyping and primer details for 058A08 - At5g51930
Line ID 058A08
Locus At5g51930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21103324 of line 058A08 Chr5:21103324
   
Insertion allele amplicon 8474 - AY16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AY16: 5'-ACGCATTATGCAGACAAGTCATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21102995
   
Insertion allele amplicon 8474 - FM36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FM36: 5'-CCTCCATGGTAATGCCATATAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21104051
   
wt amplicon (over InsPos) FM36 - AY16
wt amplicon start Chr5:21102995
wt amplicon end Chr5:21104051
wt amplicon size 1057 bp
Locus specific primer AY16: 5'-ACGCATTATGCAGACAAGTCATAC-3'
2nd locus specific primer FM36: 5'-CCTCCATGGTAATGCCATATAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11