Genotyping and primer details


Genotyping and primer details for 058E06 - At3g48990
Line ID 058E06
Locus At3g48990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18161386 of line 058E06 Chr3:18161386
   
Insertion allele amplicon 8474 - EK05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EK05: 5'-GCTCTTATCACCGCTAAAAACATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18160866
   
Insertion allele amplicon 3144 - FM37
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FM37: 5'-TATAAACAACGTCAAGCATCTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18161849
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11