Genotyping and primer details


Genotyping and primer details for 059H06 - At4g21200
Line ID 059H06
Locus At4g21200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11304898 of line 059H06 Chr4:11304898
   
Insertion allele amplicon 3144 - 41wn
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 41wn: 5'-GAAAATGGGATTTAGACCAACTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11304116
   
Insertion allele amplicon 3144 - FM38
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FM38: 5'-GAGAAAAGAAAGGAAGAGTCACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11304598
   
Insertion allele amplicon 8474 - MR99
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR99: 5'-ACCCTTTTTCCAATTTTGAGTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11305536
   
wt amplicon (over InsPos) 41wn - MR99
wt amplicon start Chr4:11304116
wt amplicon end Chr4:11305536
wt amplicon size 1421 bp
Locus specific primer MR99: 5'-ACCCTTTTTCCAATTTTGAGTCTT-3'
2nd locus specific primer 41wn: 5'-GAAAATGGGATTTAGACCAACTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11