Genotyping and primer details


Genotyping and primer details for 068A03 - At3g19590
Line ID 068A03
Locus At3g19590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6805945 of line 068A03 Chr3:6805945
   
Insertion allele amplicon 8474 - IL02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IL02: 5'-AAGAATACTCAACACATCGCACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6806449
   
wt amplicon (over InsPos) 09dd - IL02
wt amplicon start Chr3:6805570
wt amplicon end Chr3:6806449
wt amplicon size 880 bp
Locus specific primer IL02: 5'-AAGAATACTCAACACATCGCACTG-3'
2nd locus specific primer 09dd: 5'-CGTGTTTTACATGATTCATTTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11