Genotyping and primer details


Genotyping and primer details for 085D11 - At1g30440
Line ID 085D11
Locus At1g30440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10759623 of line 085D11 Chr1:10759623
   
Insertion allele amplicon 3144 - JU09
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer JU09: 5'-AAAGAGCTCAAAACCAAATTTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10760031
   
Insertion allele amplicon 3144 - PH05
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer PH05: 5'-AAAGAGCTCAAAACCAAATTTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10760031
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11