Genotyping and primer details


Genotyping and primer details for 092B09 - At1g48220
Line ID 092B09
Locus At1g48220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17803366 of line 092B09 Chr1:17803366
   
Insertion allele amplicon 8474 - 45ym
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45ym: 5'-ATGTTTGAAATCCGTATCCCTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17802525
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11