Genotyping and primer details


Genotyping and primer details for 102C11 - At2g23290
Line ID 102C11
Locus At2g23290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9905968 of line 102C11 Chr2:9905968
   
Insertion allele amplicon 8474 - 54wr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 54wr: 5'-TAAATCCGCCATGTAACTCCTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9905009
   
Insertion allele amplicon 3144 - M70b
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer M70b: 5'-AATCCAATTTAGAAATTACGCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9906471
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11