Genotyping and primer details


Genotyping and primer details for 103B06 - At2g30470
Line ID 103B06
Locus At2g30470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12982705 of line 103B06 Chr2:12982705
   
Insertion allele amplicon 8474 - ZZ16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ZZ16: 5'-TGAGAAAACTCTTAGTGCCAGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12983308
   
Insertion allele amplicon 3144 - ZZ17
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer ZZ17: 5'-CGATGGTAGGCTTTACACTAGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12982361
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11