Genotyping and primer details


Genotyping and primer details for 108C01 - At5g49945
Line ID 108C01
Locus At5g49945 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20319088 of line 108C01 Chr5:20319088
   
Insertion allele amplicon 8474 - 47cp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47cp: 5'-AATGTGTATTCGCTGGGGATATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20318504
   
wt amplicon (over InsPos) 11dy - 47cp
wt amplicon start Chr5:20318504
wt amplicon end Chr5:20319322
wt amplicon size 819 bp
Locus specific primer 47cp: 5'-AATGTGTATTCGCTGGGGATATAG-3'
2nd locus specific primer 11dy: 5'-CCTCCATCATTTTCTTCTTTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11