Genotyping and primer details


Genotyping and primer details for 114C03 - At5g58430
Line ID 114C03
Locus At5g58430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23621795 of line 114C03 Chr5:23621795
   
Insertion allele amplicon 8474 - DN35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DN35: 5'-ATACATGTTACAAATCCTCCCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23621359
   
wt amplicon (over InsPos) 07dk - DN35
wt amplicon start Chr5:23621359
wt amplicon end Chr5:23622295
wt amplicon size 937 bp
Locus specific primer DN35: 5'-ATACATGTTACAAATCCTCCCTGC-3'
2nd locus specific primer 07dk: 5'-AGTCTGTGTTCTCGGATCAGTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11