Genotyping and primer details


Genotyping and primer details for 116E01 - At3g23210
Line ID 116E01
Locus At3g23210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8284233 of line 116E01 Chr3:8284233
   
Insertion allele amplicon 8474 - H34a
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer H34a: 5'-TCCATCAATCGAAGACGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8284910
   
wt amplicon (over InsPos) 08id - H34a
wt amplicon start Chr3:8283940
wt amplicon end Chr3:8284910
wt amplicon size 971 bp
Locus specific primer H34a: 5'-TCCATCAATCGAAGACGATG-3'
2nd locus specific primer 08id: 5'-TTGTCATTTAGCTTTTCCCTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11