Genotyping and primer details


Genotyping and primer details for 120D03 - At5g11300
Line ID 120D03
Locus At5g11300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3602364 of line 120D03 Chr5:3602364
   
Insertion allele amplicon 3144 - 42ws
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 42ws: 5'-GCTTCGTAAGTCCCTACTTTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3602981
   
Insertion allele amplicon 8474 - AS03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AS03: 5'-CGCCACACAATAGCTACAGAATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3601687
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11