Genotyping and primer details


Genotyping and primer details for 125A07 - At1g16210
Line ID 125A07
Locus At1g16210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5547667 of line 125A07 Chr1:5547667
   
Insertion allele amplicon 8474 - FM86
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FM86: 5'-GAGTCTTCTGCTTTCTTAGCCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5547077
   
Insertion allele amplicon 8474 - PE38
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PE38: 5'-ACGTGAGCCACTTTATCACTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5548239
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11