Genotyping and primer details


Genotyping and primer details for 125C01 - At2g47260
Line ID 125C01
Locus At2g47260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19406741 of line 125C01 Chr2:19406741
   
Insertion allele amplicon 3144 - JA29
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer JA29: 5'-ATTCATACGTGCTTCTTTCCTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19407312
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11