Genotyping and primer details


Genotyping and primer details for 130E09 - At3g51960
Line ID 130E09
Locus At3g51960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19284055 of line 130E09 Chr3:19284055
   
Insertion allele amplicon 8474 - FL11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL11: 5'-AAAATGAGGAAGACAGGGAAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19284544
   
Insertion allele amplicon 3144 - Z24a
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer Z24a: 5'-TTGGAATCCGACCTCTATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19282753
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11