Genotyping and primer details


Genotyping and primer details for 130G11 - At2g13680
Line ID 130G11
Locus At2g13680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:5697673 of line 130G11 Chr2:5697673
   
Insertion allele amplicon 8474 - FL12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL12: 5'-AAAAACGTTTCCTTTAGCAACAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:5697161
   
Insertion allele amplicon 3144 - VL40
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer VL40: 5'-AATACTGATTAGATCAATGGGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:5698144
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11