Genotyping and primer details


Genotyping and primer details for 135A08 - At5g59670
Line ID 135A08
Locus At5g59670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24041604 of line 135A08 Chr5:24041604
   
Insertion allele amplicon 8474 - MN58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN58: 5'-TGGAACTAAGATTCATGCTGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24041002
   
Insertion allele amplicon 8474 - NR71
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NR71: 5'-GTATAGCTTCCAGTTCTCATTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24042181
   
wt amplicon (over InsPos) MN58 - NR71
wt amplicon start Chr5:24041002
wt amplicon end Chr5:24042181
wt amplicon size 1180 bp
Locus specific primer NR71: 5'-GTATAGCTTCCAGTTCTCATTGGC-3'
2nd locus specific primer MN58: 5'-TGGAACTAAGATTCATGCTGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11