Genotyping and primer details


Genotyping and primer details for 138A09 - At3g61570
Line ID 138A09
Locus At3g61570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22781984 of line 138A09 Chr3:22781984
   
Insertion allele amplicon 3144 - 04wm
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 04wm: 5'-GTTTGAGTCGTTTTAATTCTTGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22781355
   
wt amplicon (over InsPos) 29xd - 04wm
wt amplicon start Chr3:22781355
wt amplicon end Chr3:22782709
wt amplicon size 1355 bp
Locus specific primer 04wm: 5'-GTTTGAGTCGTTTTAATTCTTGCC-3'
2nd locus specific primer 29xd: 5'-GAGATGCGTTCTTAAGTGAAGGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11