Genotyping and primer details


Genotyping and primer details for 140G03 - At4g29660
Line ID 140G03
Locus At4g29660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14534751 of line 140G03 Chr4:14534751
   
Insertion allele amplicon 3144 - 55vo
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 55vo: 5'-TTATATCTGCGTATCAAAGTCGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14534428
   
Insertion allele amplicon 3144 - RK44
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer RK44: 5'-CCTTCACCATAGTCTCTGATAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14534459
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11