Genotyping and primer details


Genotyping and primer details for 140H07 - At1g04980
Line ID 140H07
Locus At1g04980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1415833 of line 140H07 Chr1:1415833
   
Insertion allele amplicon 8474 - ML13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ML13: 5'-AATATCATTGGTGCGTACACGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1416228
   
wt amplicon (over InsPos) AJ24 - ML13
wt amplicon start Chr1:1415341
wt amplicon end Chr1:1416228
wt amplicon size 888 bp
Locus specific primer ML13: 5'-AATATCATTGGTGCGTACACGTAA-3'
2nd locus specific primer AJ24: 5'-TTACCATCCAAACGATCCTTTAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11