Genotyping and primer details


Genotyping and primer details for 145E02 - At4g33060
Line ID 145E02
Locus At4g33060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15949970 of line 145E02 Chr4:15949970
   
Insertion allele amplicon 8474 - JK34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JK34: 5'-TGGAAGACAAATGATACAGAGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15950645
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 31.05.2018 12:55