Genotyping and primer details


Genotyping and primer details for 146F12 - MUF9
Line ID 146F12
Locus MUF9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24308290 of line 146F12 Chr5:24308290
   
Insertion allele amplicon 3144 - 26wp
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 26wp: 5'-AACGGGTATGATGATCTCCTTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24308948
   
Insertion allele amplicon 3144 - KR57
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KR57: 5'-AATGGCTTTCCCTCTAATTTCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24307950
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11