Genotyping and primer details


Genotyping and primer details for 150C10 - At5g40540
Line ID 150C10
Locus At5g40540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16238269 of line 150C10 Chr5:16238269
   
Insertion allele amplicon 8474 - AE08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AE08: 5'-TGGATGACTCCATGAGAGTGTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16238593
   
Insertion allele amplicon 8474 - NB97
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NB97: 5'-GTTCTGAATCCATTATTGTTTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16237815
   
wt amplicon (over InsPos) AE08 - NB97
wt amplicon start Chr5:16237815
wt amplicon end Chr5:16238593
wt amplicon size 779 bp
Locus specific primer NB97: 5'-GTTCTGAATCCATTATTGTTTCCC-3'
2nd locus specific primer AE08: 5'-TGGATGACTCCATGAGAGTGTAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11