Genotyping and primer details


Genotyping and primer details for 151F10 - At2g29990
Line ID 151F10
Locus At2g29990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12794598 of line 151F10 Chr2:12794598
   
Insertion allele amplicon 8474 - PC56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PC56: 5'-TAAAAAGGGTGTAATGGAGTGGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12794248
   
wt amplicon (over InsPos) 32dk - PC56
wt amplicon start Chr2:12794248
wt amplicon end Chr2:12795131
wt amplicon size 884 bp
Locus specific primer PC56: 5'-TAAAAAGGGTGTAATGGAGTGGTC-3'
2nd locus specific primer 32dk: 5'-AGACTTTGACTGATGGATTGAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11