Genotyping and primer details


Genotyping and primer details for 153A10 - At3g60800
Line ID 153A10
Locus At3g60800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22469204 of line 153A10 Chr3:22469204
   
Insertion allele amplicon 8474 - CB39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB39: 5'-AAAAACCACTGAGAAATAGCTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22468860
   
Insertion allele amplicon 3144 - NK21
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NK21: 5'-CCAAGGTTAAAACAAAAATTTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22469800
   
wt amplicon (over InsPos) NK21 - CB39
wt amplicon start Chr3:22468860
wt amplicon end Chr3:22469800
wt amplicon size 941 bp
Locus specific primer CB39: 5'-AAAAACCACTGAGAAATAGCTCCA-3'
2nd locus specific primer NK21: 5'-CCAAGGTTAAAACAAAAATTTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11